Ciona intestinalis NADH dehydrogenase NDX confers stress-resistance and extended lifespan on Drosophila |
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| Authors: | Dmytro V. Gospodaryov Oleh V. Lushchak Bohdana M. Rovenko Natalia V. Perkhulyn Mike Gerards Tea Tuomela Howard T. Jacobs |
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| Affiliation: | 1. BioMediTech and Tampere University Hospital, University of Tampere, FI-33014, Finland;2. Department of Biochemistry and Biotechnology, Vasyl Stefanyk Precarpathian National University, Ivano-Frankivsk 76025, Ukraine;3. Research Program of Molecular Neurology, University of Helsinki, FI-00014, Finland |
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| Abstract: | An assembled cDNA coding for the putative single-subunit NADH dehydrogenase (NDX) of Ciona intestinalis was introduced into Drosophila melanogaster. The encoded protein was found to localize to mitochondria and to confer rotenone-insensitive substrate oxidation in organello. Transgenic flies exhibited increased resistance to menadione, starvation and temperature stress, and manifested a sex and diet-dependent increase in mean lifespan of 20–50%. However, NDX was able only weakly to complement the phenotypes produced by the knockdown of complex I subunits. |
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| Keywords: | Alternative respiratory chain enzymes Tunicate Gene therapy Oxidative stress Ageing |
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