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Sixty New STSs (Sequence-Tagged Sites) of Human Chromosome 21 |
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| Authors: | Tanahashi Hiroshi; Ito Takashi; Hattori Masahira; Ohira Miyuki; Ohki Misao; Tashiro Kosuke; Sakaki Yoshiyuki |
| Institution: | 1Human Genome Center, Institute of Medical Science, University of Tokyo Shirokanedai, Minato-ku, Tokyo 108, Japan 2National Cancer Center Institute Tsukiji, Chuo-ku, Tokyo 104, Japan 3Kazusa DNA Research Institute 473-2 Ookanazawa-cho, Midori-ku, Chiba 260, Japan 4Faculty of Science, University of Tokyo Hongo, Bunkyo-ku, Tokyo 113, Japan |
| Abstract: | From human chromosome 21-specific libraries, 22 SfiI linkingclones and 38 P1 clones were isolated and regionally mappedon the chromosome. The terminal sequences of these clones weredetermined and pairs of PCR primers were generated which couldspecifically amplify the sequenced regions. These sequence-taggedsites (STSs) should be useful for constructing a high resolutionmap of human chromosome 21. |
| Keywords: | human chromosome restriction enzyme SfiI linking clone P1 clone high-resolution physical map PCR |
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