Selection of Peptides for Lipopolysaccharide Binding on to Epoxy Beads and Selective Detection of Gram-negative Bacteria |
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| Authors: | Yun-Gon Kim Chang-Soo Lee Woo-Jae Chung Eun-Mi Kim Dong-Sik Shin June-Hyung Kim Yoon-Sik Lee Junho Chung Byung-Gee Kim |
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| Affiliation: | (1) Program in Biochemical Engineering and Biotechnology, Seoul National University, San56–1, Sillim-Dong, 151-742 Gwanak-Gu, Seoul, Korea;(2) Department of Chemical Engineering, Chungnam National University, 305-364 Daejeon, Korea;(3) School of Chemical and Biological Engineering, Seoul National University, 151-742 Seoul, Korea;(4) Department of Biochemistry and Molecular Biology and Cancer Research Institute, Seoul National University College of Medicine, 110-799 Seoul, Korea |
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| Abstract: | Lipopolysaccharide (LPS)-binding peptides were enriched by using epoxy beads as a novel support to immobilize LPS for a phage displayed peptide library screening. The sequence of Phe-Ala-Pro-Trp (FAPW) was the most significant consensus motif of 10 selected clones, and Pro-Phe (PF) was the key dipeptide for binding at the apex of the loop to form a characteristic structure of CXXPFXXXC. Moreover, AWLPWAK, one of the highly conserved heptamer peptides, could detect specifically Gram-negative bacteria via a whole cell binding test at 106 cells ml−1. Received 12 July 2005; Revisions requested 1 August 2005 and 26 September 2005; Revisions received 12 September 2005 and 25 October 2005; Accepted 1 November 2005 |
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| Keywords: | bacteria detection epoxy beads lipopolysaccharide peptides phage display |
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