| 丁酸钠对人胃腺癌SGC-7901细胞的生物学效应 |
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| 引用本文： | 吕桂芝,高燕,黄衍川,林仲翔,汪堃仁. 丁酸钠对人胃腺癌SGC-7901细胞的生物学效应[J]. 分子细胞生物学报, 1989, 0(2) |
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| 作者姓名： | 吕桂芝 高燕 黄衍川 林仲翔 汪堃仁 |
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| 作者单位： | 北京肿瘤防治研究所细胞生物学室(吕桂芝，高燕，黄衍川，林仲翔)，北京肿瘤防治研究所细胞生物学室(汪堃仁) |
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| 摘 要： | 应用兔抗纤维粘(连)蛋白(FN)抗体和兔抗3t,5,环腺苷酸(cAMP)特异性抗体,并用羊抗兔IgG荧光抗体,在荧光显微镜下观察丁酸钠对人胃腺癌SGC一7901细胞系的纤维粘(连)蛋白(FN)和cAMP水平的变化。实验结果表明丁酸钠有增强SGC一7901细胞内纤维粘(连)蛋白(FN)和c.~MP水平的作用;应用电镜细胞化学和扫描电镜的技术方法,观察到人胃腺癌SGC一7901细胞在丁酸钠作用下膜表而(Na 一K )一ATP酶活性明显下降;微绒毛明显减退,膜表面较光滑。这可能说明丁酸钠通过抑制(Na~一K )一ATP酶活性,提高了细咆内cAMP水平,调控细胞的增殖和分化。
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| 关 键 词： | 人胃腺癌SGC-7901细胞 丁酸钠 纤维粘(连)蛋白(FN) 3' 5'环腺苷酸(cAMP) (Na~ -K~ )-ATP酶 细胞的增殖和分化 |
THE BIOLOGICAL EFFECT OF SODIUM BUTYRATE (NaBT) ON SGC-7901 CELLS |
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| Abstract: | Changes of (Na~ -K~ )-ATPase activity,cAMP and fibronectin (FN) content and cellsurface microvilli were studied cytochemically,immunocytochemically and scanning elec-tron microscopically on human stomachGlandular carcinoma (SGC-7901) cells trea-ted with NaBT(2.5mM). It was found that NaBT not only inhi-bited cell growth but also remarkably decrea-sed the activity of cell surface (Na~ -K~ )-ATPase of SGC-7901 cells.Note worthy wasthat,in comparison with the untreated tumorcells,the increase of the intensity of intracel-lular cAMP and FN immunofluorescence inNaBT-treated tumor cells was striking.More-over,in contrast to untreated tumor cells,the cell surface of NaBT-treated tumor cellsshowed more smooth and fewer microvilliunder SEM. That NaBT may induce differentiation ofSGC-7901 cells through inhibition of (Na~ -K~ )-ATPase activity and modulation of cel-lular cAMP and FN content was discussed. |
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| Keywords: | Human stomach glandular carcinoma cells.Sodium Butyrate.(Na~ -K~ )-ATPase.cAMP.Fibronectin.Microvilli. |
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