Electrokinetic properties of asparaginase sensitive or resistant mouse leukemic cells treated with L-asparaginase |
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Authors: | HBruce Bosmann David Kessel |
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Institution: | Department of Pharmacology and Toxicology and Division of Oncology, University of Rochester School of Medicine and Dentistry, Rochester, N.Y. 14642 U.S.A. |
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Abstract: | The electrophoretic mobility of L5178Y cells in 0.0145 M NaCl, 4.5% sorbitol, 0.6 mM NaHCO3, pH 7.2, at 25°C was — 1.78 μ·s?1·V?1·cm?1 while that of an L-asparaginase resistant subline, L5178Y/ASN, was — 1.11 μm·s?1·V?1·cm?1. Both cell lines were characterized by terminal sialic acid residues on their surfaces. Treatment of L5178Y cells for 90 min with 10 units of L-asparaginase per ml in saline decreased the electrophoretic mobility of the cells to — 1.65 μm·s?1·V?1·cm?1 while treatment in Fischer's medium decreased the mobility to — 1.25 μm·s?1·V?1·cm?1; neither treatment had a significant effect on the L5178Y/ASN electrophoretic mobility. The results suggest that L-asparaginase has an immediate and specific effect on synthesis of cell surface asparaginyl glycoproteins. |
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