Fractionation and properties of four heparitin sulfates from beef lung tissue: Isolation and partial characterization of a homogeneous species of heparitin sulfate |
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Authors: | CarlP Dietrich Helena B Nader |
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Institution: | Departamento de Bioquimica e Farmacologia Escola Paulista de Medicina Caixa Postal 20.372, Sao Paulo, SP, Brazil |
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Abstract: | Several commerical batches of heparitin sulfate extracted from beef lung tissue were fractionated into at least four distinct mucopolysaccharides by a combination of polyacrylamide and agarose gel electrophoresis. The four heparitin sulfates (A, B, C and D) were distinguished from each other and from heparin by several physical and chemical properties such as electrophoretic migration, molecular weight, presence of N-acetyl, N- and )-sulfate residues, optical rotation and enzymatic degradation. Of particular significance was the isolation of a heparitin sulfate (heparitin sulfate C) with a homogeneous molecular weight. |
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Keywords: | TEMED |
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