Inhibition of muscarinic receptor-mediated Ca27#x002B; mobilization by phorbol 12-myristate 13-acetate in chick embryo cells |
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Authors: | Heinrich Schmidt Günter Oettling Ulrich Drews |
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Affiliation: | (1) Anatomisches Institut der Universität Tübingen, Österbergstraße 3, D-7400 Tübingen, Germany |
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Abstract: | Summary A muscarinic cholinergic receptor is present on undifferentiated cells of the chick embryo. Stimulation of the muscarinic receptor with muscarinic agonists triggers intracellular Ca2#x002B; mobilization. Here, we investigate the effect of phorbol 12-myristate 13-acetate (PMA) on the muscarinic receptor-mediated Ca2#x002B; mobilization, which is monitored in cell suspensions of chick embryos of stage 24 by chlorotetracycline fluorescence. PMA inhibits the Ca2#x002B; mobilization in a time-dependent and concentration-dependent manner without changing the ED50 of acetylcholine. The concentration of PMA that gives halfmaximal inhibition is 3.1×10–9 M PMA. |
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Keywords: | Phorbol ester Muscarinic receptor Calcium Chick embryo Morphogenesis |
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