Studies on Haematococcus pluvialis for improved production of astaxanthin by mutagenesis |
| |
| Authors: | Tripathi Usha Venkateshwaran G Sarada R Ravishankar GA |
| |
| Institution: | (1) Plant Cell Biotechnology Department, Central Food Technological Research Institute, Mysore–, 570 013, India;(2) Food Microbiology Department, Central Food Technological Research Institute, Mysore–, 570 013, India |
| |
| Abstract: | Cultures of Haematococcus pluvialis were exposed to mutagens like u.v. and EMS (ethyl methanesulphonate). The results showed that the survival rate decreased with the increase in u.v. exposure time and increase in EMS concentration. These mutants were further screened using inhibitors of the carotenoid biosynthetic pathway viz. diphenylamine (15–90  M), nicotine (160–320 M) and compactin (1.5–3.0 M). The mutants thus obtained showed early enhanced (2.2–3.2-fold) astaxanthin accumulation and also exhibited higher lycopene cyclase activity. |
| |
| Keywords: | Mutagens ethyl methanesulphonate Haematococcus pluvialis diphenylamine nicotine compactin salinity stress |
| 本文献已被 SpringerLink 等数据库收录！ |
