Influence of Flammulina velutipes mycelia culture conditions on antimicrobial metabolite production |
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Authors: | Marcelo Rodrigues de Melo Luzia Doretto Paccola-Meirelles Terezinha de Jesus Faria Noemia Kazue Ishikawa |
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Affiliation: | 1.Departamento de Biologia Geral, Centro de Ciências Biológicas,Universidade Estadual de Londrina,Londrina, Paraná,Brazil;2.Departamento de Química, Centro de Ciências Exatas,Universidade Estadual de Londrina,Paraná,Brazil |
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Abstract: | Enokipodins A, B, C, and D are α-cuparene-type sesquiterpenoids antimicrobial metabolites produced in the stationary stage of Flammulina velutipes mycelia development in malt extract broth. This study assessed the influence of nutritional and environmental factors on F. velutipes mycelia culture for the production of these metabolites. The mycelia growth and antimicrobial activity were assessed by determining dry matter and the diffusion in agar method, respectively. The best F. velutipes mycelia growth was observed in dextrose potato broth, and greater antimicrobial metabolite production occurred in complete Pontecorvo’s culture medium. Environmental modifications, such as a rise in temperature from 25° to 37°C on the 15th day of F. velutipes mycelia culture in malt extract and peptone broth, also optimized antimicrobial metabolite production. The metabolites produced in these treatments were correlated with the enokipodins A and B in thin-layer chromatography (TLC) and the antifungal activity test by TLC bioautography. This study showed that there was no correlation between biomass production and antimicrobial metabolite production, but there may be a correlation between culture medium composition and enokipodins biosynthesis. |
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Keywords: | Basidiomycetes Cuparene Enokipodins A-D Enokitake Sesquiterpenoid |
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