首页 | 本学科首页   官方微博 | 高级检索  
   检索      


Characterization of an echinocandin B-producing strain blocked for sterigmatocystin biosynthesis reveals a translocation in the stcW gene of the aflatoxin biosynthetic pathway
Authors:R L Hodges  H S Kelkar  X Xuei  P L Skatrud  N P Keller  T H Adams  R E Kaiser  V A Vinci  D McGilvray
Institution:(1) Natural Products Research and Development, Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, IN 46285,;(2) Department of Genetics, University of Georgia, C330 Life Sciences Bldg., Athens, GA 30602,;(3) Department 4PN, Abbott Laboratories, 100 Abbott Park Road, Building J35, Abbott Park, IL 60064,;(4) Infectious Diseases Research, Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, IN 46285,;(5) Department of Plant Pathology and Microbiology, Texas A&M University, College Station, TX 77843,;(6) Mystic Research Center, 62 Maritime Drive, Mystic, CT 06355,;(7) Bio-process Analytical Development, Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, IN 46285 ,
Abstract:Echinocandin B (ECB), a lipopolypeptide used as a starting material for chemical manufacture of the anti-Candida agent LY303366, is produced by fermentation using a strain of Aspergillus nidulans. In addition to ECB, the wild-type strain also produces a significant level of sterigmatocystin (ST), a potent carcinogen structurally related to the aflatoxins. Characterization of a mutant designated A42355-OC-1 (OC-1), which is blocked in ST biosynthesis, was the result of a chromosomal translocation. The chromosomal regions containing the breakpoints of the translocation were isolated and DNA sequencing and PCR analysis of the chromosomal breakpoints demonstrated the translocation occurred within the stcW gene of the ST biosynthetic pathway, resulting in disruption of the open reading frame for this gene. Biochemical feeding studies indicate the involvement of this gene product in the conversion of averufin to 1-hydroxy versicolorone. This work demonstrates an effective synergy between classical strain improvement methods and molecular genetics. Journal of Industrial Microbiology & Biotechnology (2000) 25, 333–341. Received 27 April 2000/ Accepted in revised form 25 November 2000
Keywords:: Aspergillus nidulans  aflatoxin  sterigmatocystin  mutagenesis  translocation  echinocandin B
本文献已被 PubMed SpringerLink 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号