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| 降钙素基因对胡萝卜的遗传转化 | |
| 引用本文： | 张丽华,程智慧,陈杭,赵吉强,李霞.降钙素基因对胡萝卜的遗传转化[J].生物技术通讯,2005,16(5):512-514. |
| 作者姓名： | 张丽华 程智慧 陈杭 赵吉强 李霞 |
| 作者单位： | 1. 北京市农林科学院,蔬菜研究中心,北京,100089;西北农林科技大学,园艺学院,陕西,杨凌,712100 2. 西北农林科技大学,园艺学院,陕西,杨凌,712100 3. 北京市农林科学院,蔬菜研究中心,北京,100089 |
| 摘 要： | 以新透心红胡萝卜为植物材料，用微弹轰击法对胡萝卜愈伤组织进行了降钙素基因的遗传转化。对所得再生植株进行了PCR检测及Southern印迹检测。在16棵Kan抗性株中有13棵PCR阳性株；4棵存活的PCR阳性株经Southern印迹检测，有3株为阳性。对收获的2个肉质根做PCR检测，均为阳性；对其中较大的肉质根作Southern印迹检测，结果也为阳性。这些检测结果说明降钙素基因被成功地整合到胡萝卜基因组中。 |
| 关 键 词： | 胡萝卜 降钙素 遗传转化 |
| 文章编号： | 1009-0002(2005)05-0512-03 |
| 收稿时间： | 01 13 2005 12:00AM |
| 修稿时间： | 2005年1月13日 |
Transformation of calcitonin gene to carrot |
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| ZHANG Li-hua,CHENG Zhi-hui,CHEN Hang,ZHAO Ji-qiang,LI Xia.Transformation of calcitonin gene to carrot[J].Letters in Biotechnology,2005,16(5):512-514. | |
| Authors: | ZHANG Li-hua CHENG Zhi-hui CHEN Hang ZHAO Ji-qiang LI Xia |
| Institution: | 1. Vegetable Center of Beijing Academy of Agrieuhure and Forestry Science, Beijing 100089; 2. College of Horticulture Science, Northwest Science and Technology University of Agrieuhure and Forestry, Yangling 712100, China |
| Abstract: | Carrot cultivar 'Xintouxinhong' was transformed with calcitonin gene by micro-bombardment. Among 16 Kanamycin-resistant plants, 13 were positive by PCR detection. 3 plants were positive by Southern blot detection in 4 living plants. Two fleshy roots were harvested in the 3 positive plants and they showed positive in PCR detection. Southern blotting of the bigger one fleshy root showed it was positive. The results indicated that the calcitonin gene had been inserted into the carrot genome DNA. |
| Keywords: | carrot calcitonin gene transformation |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |