Enhancement of tyrosinase inhibition of the extract of Veratrum patulum using cellulase |
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Authors: | Kim Dong Hyun Kim Jin Hee Baek Seung Hwa Seo Jin Ho Kho Yung Hee Oh Tae Kwang Lee Choong Hwan |
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Institution: | Korea Research Institute of Bioscience and Biotechnology, Daejon 305-333. |
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Abstract: | Inhibitors of melanin biosynthesis were screened by using three different methods. The extract of Veratrum patulum contains hydroxystilbene compounds that are potent tyrosinase inhibitors. We evaluated the enzyme inhibitory property on the mushroom tyrosinase of hydroxystilbene compounds including resveratrol, oxyresveratrol, and their analogs. Biotransformation using cellulase of the whole extract brought about an increase in the inhibitory activity of the products on mushroom tyrosinase. The enhancement of tyrosinase inhibition is supposed to increase the concentration of aglycon, which has superior inhibitory activity to its glycoside. Eventually, melanin biosynthesis was inhibited by the enhanced tyrosinase inhibitory activity of the extract. This result indicated that deglycosylation of stilbene compounds has exerted more effective inhibition on the enzyme than that of the unprocessed plant extract. |
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Keywords: | biotransformation cellulase tyrosinase inhibitor resveratrol Veratrum patulum |
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