Optical chamber system designed for microscopic observation of living cells under extremely high hydrostatic pressure |
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Authors: | Sumihiro Koyama Tetsuya Miwa Takako Sato Masuo Aizawa |
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Affiliation: | Frontier Research System for Extremophiles, Japan Marine Science and Technology Center, Yokosuka. skoyama@jamstec.go.jp |
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Abstract: | A novel pressure chamber system has been developed for the study of living cells under conditions of extremely high hydrostatic pressure up to 100 MPa (1 atm = 0.101325 MPa). The temperature in the chamber is thermostatically controlled in the range from 2 degrees to 80 degrees C. Two high-pressure pumps are employed for continuous perfusion of the chamber with culture medium and a chemical solution under high hydrostatic pressure conditions. The chamber has a 2-mm-thick glass window 2 mm in diameter, with a minimum working distance of 3.8 mm. The chamber system is designed to be adaptable to a variety of microscopic and imaging techniques. Using this chamber system, we successfully carried out real-time observations of elongated Escherichia coli and rounded HeLa cells under pressure. |
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