Mass isotopomer study of anaplerosis from propionate in the perfused rat heart |
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Authors: | Kasumov Takhar Cendrowski Andrea V David France Jobbins Kathryn A Anderson Vernon E Brunengraber Henri |
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Institution: | Department of Nutrition, Case Western Reserve University, School of Medicine - WG 48, 10900 Euclid Avenue, Cleveland, OH 44106-4954, USA. |
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Abstract: | Anaplerosis from propionate was investigated in rat hearts perfused with 0-2mM (13)C(3)]propionate and physiological concentrations of glucose, lactate, and pyruvate. The data show that when the concentration of (13)C(3)]propionate was raised from 0 to 2mM, total anaplerosis increased from 5% to 16% of the turnover of citric acid cycle intermediates. Then, (13)C(3)]propionate abolished anaplerosis from endogenous substrates, glucose, lactate, and pyruvate. Also, while the contents of propionyl-CoA and methylmalonyl-CoA increased with (13)C(3)]propionate concentration, the content of succinyl-CoA decreased, presumably via activation of succinyl-CoA hydrolysis by a decrease in free CoA. Under our conditions, (13)C(3)]propionate was a purely anaplerotic substrate since there was no labeling of mitochondrial acetyl-CoA, reflected by the labeling of the acetyl moiety of citrate. |
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Keywords: | Anaplerosis Citric acid cycle Mass isotopomer distribution Cardiac metabolism |
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