Developmental change of protein constituents in chicken gizzards |
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Authors: | S Hirai T Hirabayashi |
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Affiliation: | Institute of Biological Sciences, The University of Tsukuba Niihari-gun Sakura-mura, Ibaraki 305, Japan |
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Abstract: | Developmental change of protein constituents of chick gizzard smooth muscle was described by the fluorescent antibody technique and two-dimensional polyacrylamide gel electrophoresis. Myosin heavy chain, tropomyosin, and desmin were immunohistologically detected in 5-day-old gizzard primordia, but myoglobin was detected after 19 days of incubation. Two-dimensional polyacrylamide gel electrophoresis revealed that most structural proteins including beta- and gamma-actin are synthesized almost simultaneously in the primordium, and accumulate in three patterns by which the proteins examined are classified: (1) gradually increasing protein (gamma-actin, tropomyosin, desmin), (2) abruptly increasing protein at a certain stage (myosin, myoglobin), (3) decreasing or constantly kept protein (tubulin, beta-actin). Based on the quantitative analysis of protein constituents, the nature of regulatory system of protein synthesis in smooth muscle and the possible functional difference between beta- and gamma-actin are discussed. |
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Keywords: | To whom all correspondence should be addressed: Institute of Biological Sciences The University of Tsukuba Niihari-gun Sakura-mura Ibaraki 305 Japan. |
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