Evidence of an association between poly(3-hydroxybutyrate) accumulation and phosphotransbutyrylase expression in<Emphasis Type="Italic"> Bacillus megaterium</Emphasis> |
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Authors: | Gustavo?J?Vazquez M?Julia?Pettinari Email author" target="_blank">Beatriz?S?MéndezEmail author |
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Institution: | (1) Departamento de Química Biológica, Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Buenos Aires, Argentina;(2) Present address: Microbiology Laboratory, Brookhaven National Laboratory, Environmental Sciences Department, Upton, New York, USA |
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Abstract: | Molecular analysis of a genomic region of Bacillus megaterium, a polyhydroxybutyrate (PHB)-producing microorganism, revealed the presence of a gene coding for the enzyme phosphotransbutyrylase (Ptb). Enzyme activity was measured throughout the different growth phases of B. megaterium and was found to correlate with PHB accumulation during the late-exponential growth phase. Ptb expression was repressed by glucose and activated by the branched amino acids isoleucine and valine. Overexpression of ActBm, a 54 regulator from B. megaterium whose gene is located upstream from ptb, caused an increase in Ptb activity and PHB accumulation in B. megaterium. |
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Keywords: | Bacillus megaterium Polyhydroxybutyrate Phosphotransbutyrylase |
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