Copper redistribution in Atox1-deficient mouse fibroblast cells |
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Authors: | Reagan McRae Barry Lai Christoph J Fahrni |
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Institution: | (1) School of Chemistry and Biochemistry, Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, 901 Atlantic Drive, Atlanta, GA, 30332, USA;(2) X-ray Science Division, Advanced Photon Source, Argonne National Laboratory, Argonne IL, 60439, USA; |
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Abstract: | Quantitative synchrotron X-ray fluorescence (SXRF) imaging of adherent mouse fibroblast cells deficient in antioxidant-1 (Atox1),
a metallochaperone protein responsible for delivering Cu to cuproenzymes in the trans-Golgi network, revealed striking differences in the subcellular Cu distribution compared with wild-type cells. Whereas the
latter showed a pronounced perinuclear localization of Cu, the Atox1-deficient cells displayed a mostly unstructured and diffuse
distribution throughout the entire cell body. Comparison of the SXRF elemental maps for Zn and Fe of the same samples showed
no marked differences between the two cell lines. The data underscore the importance of Atox1, not only as a metallochaperone
for delivering Cu to cuproenzymes, but also as a key player in maintaining the proper distribution and organization of Cu
at the cellular level. |
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Keywords: | |
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