Molecular monitoring of the developmental bacterial community in the gastrointestinal tract of Japanese infants |
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| Authors: | Songjinda Prapa Nakayama Jiro Kuroki Yumiko Tanaka Shigemitsu Fukuda Sanae Kiyohara Chikako Yamamoto Tetsuro Izuchi Kunio Shirakawa Taro Sonomoto Kenji |
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| Institution: | Laboratory of Microbial Technology, Division of Microbial Science and Technology, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University, Fukuoka, Japan. |
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| Abstract: | The dynamics of the developmental bacterial community in the Japanese neonatal gastrointestinal tract were examined by monitoring 16S ribosomal RNA gene (rDNA) diversity in fecal samples by PCR and denaturing gradient gel electrophoresis (DGGE). The results showed a certain pattern common in infants without antibiotic treatment, in which aerobes, e.g., Pseudomonas, appeared first and were then immediately replaced by facultative anaerobe, Enterococcus, Streptococcus, and Enterobacteriaceae through the first month, and finally strictly anaerobic Bifidobactrerium appeared. |
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