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Effects of Ammonia on Respiration, Adenylate Levels, Amino Acid Synthesis and CO2 Fixation in Cells of Chlorella vulgaris 11h in Darkness |
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| Authors: | Ohmori, Masayuki Miyachi, Shizuko Okabe, Kei-ichiro Miyachi, Shigetoh |
| Affiliation: | 1Ocean Research Institute, University of Tokyo Nakano-ku, Tokyo 164, Japan 2Radioisotope Centre, University of Tokyo Bunkyo-ku, Tokyo 113, Japan 3Institute of Applied Microbiology, University of Tokyo Bunkyo-ku, Tokyo 113, Japan |
| Abstract: | The effects of NH4Cl on respiration, adenylate and free aminoacid levels as well as dark CO2 fixation were investigated usingnitrogen-starved Chlorella vulgaris 11h cells with or withoutaddition of methionine sulfoximine (MSX), an inhibitor of glutaminesynthetase. Upon addition of NH4Cl (1 mM) to the cells not treatedwith MSX, respiration was stimulated and the level of ATP droppedrapidly, while the levels of ADP and AMP increased. NH4Cl alsostimulated amino acid synthesis, especially of glutamine, andmarkedly enhanced dark CO2 fixation. Addition of NH4Cl to MSX-treatedcells stimulated respiration and lowered the level of ATP, butdid not enhance glutamine synthesis and only slightly stimulateddark CO2 fixation. 4On leave from Institute of Medical Science, Advance R &D Co. Minami-Hashimoto, Sagamihara, Kanagawa-ken 220, Japan (Received January 28, 1984; Accepted April 19, 1984) |
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