Tolerance of equine strongylid larvae to desiccation and freezing

Some third-stage equine strongylid larvae survived freezing in water at a controlled rate of l °C/min from 10 to ?30 °C followed by immersion in liquid nitrogen and subsequent rapid thawing to 38 °C, The addition of glycerol in concentrations of 5, 10, and 20% enhanced the survival of larvae. Freezing did not affect the viability of desiccated larvae. A low percentage of larvae suspended in water survived direct immersion in liquid nitrogen.