Ethanol from hydrolyzed whey permeate using Saccharomyces cerevisiae in a membrane recycle bioreactor |
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| Authors: | Dr. M. A. Mehaia Dr. M. Cheryan |
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| Affiliation: | (1) 110 Agricultural Bioprocess Laboratory, University of Illinois, 1302 West Pennsylvania Avenue, 61801 Urbana, Illinois, USA;(2) College of Agriculture and Veterinary Medicine Dairy Technology Laboratory, King Saud University, Buriedah, Saudi Arabia |
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| Abstract: | A diauxic fermentation was observed during batch fermentation of enzyme-hydrolyzed whey permeate to ethanol by Saccharomyces cerevisiae. Glucose was consumed before and much faster than galactose. In the continuous membrane recycle bioreactor (MRB), sugar utilization was a function of dilution rate and concentration of sugars. At a cell concentration of 160 kg/m3, optimum productivity was 31 kg/(m3 · h) at ethanol concentration of 65 kg/m3. Low levels of acetate (0.05–0.1 M) reduced cell growth during continuous fermentation, but also reduced galactose utilization. |
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