First experiences with semi-autonomous robotic harvesting of protein crystals |
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Authors: | Robert Viola Jace Walsh Alex Melka Wesley Womack Sean Murphy Alan Riboldi-Tunnicliffe Bernhard Rupp |
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Affiliation: | (1) Square One Systems Design, Jackson, WY 83002, USA;(2) The Johns Hopkins University Applied Physics Laboratory, Laurel, MD 20723, USA;(3) Australian Synchrotron, Clayton, VIC, 3168, Australia;(4) k.-k. Hofkristallamt, Livermore, CA 94551, USA; |
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Abstract: | The demonstration unit of the Universal Micromanipulation Robot (UMR) capable of semi-autonomous protein crystal harvesting has been tested and evaluated by independent users. We report the status and capabilities of the present unit scheduled for deployment in a high-throughput protein crystallization center. We discuss operational aspects as well as novel features such as micro-crystal handling and drip-cryoprotection, and we extrapolate towards the design of a fully autonomous, integrated system capable of reliable crystal harvesting. The positive to enthusiastic feedback from the participants in an evaluation workshop indicates that genuine demand exists and the effort and resources to develop autonomous protein crystal harvesting robotics are justified. |
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