Isolation of canine rotavirus and study of its immunobiological properties]

Canine rotavirus was isolated in MA104 roller culture of rhesus macaque cells. Two passages in gnotobiotic puppies and two in colostrum-free puppies resulted in isolation of strain P of canine rotavirus. After 20 passages in MA104 culture the virus was adapted to MDCK culture. Optimal conditions for accumulation of canine rotavirus and its antigen (9.01 g TCD50/ml) in MDCK culture are trypsin pretreatment of the virus inoculate in the final concentration of 50 mcg/ml for 30 min at 37 degrees C, presence of trypsin (10 mcg/ml) in the maintenance medium, multiplicity of infection 0.1 TCD50/ml, and incubation in roller culture at 37 degrees C during 24-30 h. After 60 passages in cell culture, canine rotavirus completely lost its virulence for colostrum-free puppies but retained antigenic activity and induced manifest seroconversion in infected.