Structure and heterogeneity of the O-antigen chain of Salmonella Agona lipopolysaccharide |
| |
Authors: | Szafranek Janusz Kumirska Jolanta Czerwicka Małgorzata Kunikowska Danuta Dziadziuszko Halina Głośnicka Renata |
| |
Affiliation: | Department of Chemistry, University of Gda?sk, Gda?sk, Poland. janat@chem.univ.gda.pl |
| |
Abstract: | Lipopolysaccharide of Salmonella Agona smooth-type cells was obtained from bacteria by a hot phenol-water extraction procedure. Mild acid hydrolysis of lipopolysaccharide, followed by gel filtration, yielded the pure O-polysaccharide. Abequose, rhamnose, mannose, galactose and glucose in the molar ratio 0.8 : 1.0 : 1.0 : 1.1 : 0.5 were detected, and their linkages were established. Sugar configurations were determined by gas chromatography. Two repeating units, namely -->2)-[alpha-Abep-(1-->3)-]-alpha-d-Manp-(1-->4)-alpha-l-Rhap-(1-->3)-alpha-d-Galp-(1-->and -->2)-[alpha-Abep-(1-->3)-]-alpha-d-Manp-(1-->4)-alpha-l-Rhap-(1-->3)-[alpha-d-Glcp-(1-->4)-]-alpha-d-Galp-(1-->, were deduced from nuclear magnetic resonance studies. The effort to separate them was unsuccessful. An immunochemical test performed by means of Western blotting with anti O12 serum demonstrated that glucose was present in the longer lipopolysaccharide chains, at some distance from the core region. |
| |
Keywords: | Salmonella Agona lipopolysaccharide O-antigen repeating oligosaccharide structures LPS heterogeneity |
本文献已被 PubMed 等数据库收录! |
|