| 猪雌激素受体基因（ESR）一个新多态位点的发现 |
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| 引用本文： | 张冬杰,杨国伟,刘娣.猪雌激素受体基因（ESR）一个新多态位点的发现[J].遗传,2006,28(1):36-38. |
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| 作者姓名： | 张冬杰 杨国伟 刘娣 |
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| 作者单位： | （1．东北农业大学动物科学技术学院，哈尔滨 150030；2．黑龙江省农业经济职业技术学校，牡丹江 157041； |
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| 摘 要： | ESR基因是影响猪产仔数的主效基因，而且与猪的生长发育性状及胴体性状之间不存在负的基因多效性影响。目前对它的研究大都局限于Rothschild等人发现的PvuⅡ酶切位点。本实验采用PCR-SSCP方法，对ESR基因的外显子7进行检测，发现了一个新多态性位点，得到3种基因型，可以作为一个新的标记位点进一步研究。
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| 关 键 词： | 猪 ESR基因 产仔数性状 PCR-SSCP |
| 文章编号： | 0253-9772(2006)01-0036-03 |
| 收稿时间： | 2004-09-24 |
| 修稿时间： | 2005-03-13 |
A New Polymorphic Site of ESR Gene in Pigs |
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| ZHANG Dong-Jie,YANG Guo-Wei,LIU Di.A New Polymorphic Site of ESR Gene in Pigs[J].Hereditas,2006,28(1):36-38. |
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| Authors: | ZHANG Dong-Jie YANG Guo-Wei LIU Di |
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| Institution: | (1. College of Animal Science and Technology, North East Agriculture University, Harbin 150030; 2. HeiLongJiang Agricultural Economy Professional College, Mudanjiang 157041; HeiLongJiang Academy of Agricultural Sciences, Harbin 150086) |
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| Abstract: | ESR (Estrogen Receptor) gene has been determined to be one of the major genes affecting phenotype of litter size without any genetic negative correlation to growth and carcass trait. Recent studies mainly focused on the PvuII enzyme site found by Rothschild in 1996. An optimized standard PCR-SSCP protocol was employed to detect the exon 7 of ESR gene. The results showed that there were a new mutation site and three genotypes, which may be used as a new DNA marker site to further research. |
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| Keywords: | PCR-SSCP |
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