Spondias purpurea Exudate polysaccharide as affinity matrix for the isolation of a galactose-binding-lectin |
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Authors: | Daniele MA Teixeira Renata C Braga Ana CG Horta Renato A Moreira Ana CF de Brito Jeanny S Maciel Judith PA Feitosa Regina CM de Paula |
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Institution: | aDepartamento de Bioquı´mica e Biologia Molecular, Universidade Federal do Ceará, Caixa Postal 6020, CEP 60451-980, Brazil bDepartamento de Quı´mica Orgânica e Inorgânica, Universidade Federal do Ceará, Caixa Posta 12200, CEP 60455-760, Fortaleza-Ceará, Brazil |
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Abstract: | Spondias purpurea L., popularly known as ciriguela, is native and widespread tree from Mexico through Northern Peru and Brazil, particularly in semi-arid zones. This tree exudes a water soluble polysaccharide, constituted of a (1→3) linked galactan backbone substituted at C6 with d-galactose, d-xylose, l-arabinose, l-rhamnose and glucuronic acid units. Brazilian polysaccharide differs from Venezuelan on the amount of acid and arabinose as well as the presence of fucose and glucose as minor sugar. The d-galactose substitution (1→6) confers to the polysaccharide the peculiar capacity of binding -d-galactose specific lectins after cross-linking with epichlorohydrin. The gel obtained was able to specifically retain d-galactose-binding-lectins, among with those from Artocarpus incisa, Artocarpus integrifolia, Erythrina velutina and Ricinus communis. On the other hand, no glucose-binding-lectins were retained. |
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Keywords: | Polysaccharide Affinity chromatography Lectins Spondias purpurea |
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