An LED-based UV-B irradiation system for tiny organisms: System description and demonstration experiment to determine the hatchability of eggs from four Tetranychus spider mite species from Okinawa |
| |
| Affiliation: | 1. Faculty of Agriculture, Ibaraki University, Ami, Ibaraki 300-0393, Japan;2. Faculty of Agriculture, Aristotle University of Thessaloniki, 541 24 Thessaloniki, Greece;3. Center for Environment, Health and Field Sciences, Chiba University, Kashiwa, Chiba 277-0882, Japan;4. Graduate School of Agricultural Science, Kobe University, Nada, Kobe 657-8501, Japan;5. Japan Society for the Promotion of Science, Chiyoda, Tokyo 102-0083, Japan;6. Ishigaki Branch, Okinawa Prefectural Agricultural Research Center, Ishigaki, Okinawa 907-0003, Japan;1. Institute of Oncology, Tel Aviv Sourasky Medical Center, Weizmann St 6, Tel Aviv, Israel;2. Sackler Faculty of Medicine, Tel Aviv University, Chaim Levanon St 30, Tel Aviv, Israel;3. Princess Margaret Cancer Centre, University of Toronto, 610 University Ave, Toronto, Canada;4. Department of Oncology, Hospital de la Santa Creu i Sant Pau, Institut d’Investigació Biomèdica Sant Pau and Universitat Autònoma de Barcelona, Carrer de Sant Quintí 89, Barcelona, Spain;5. Institute of Oncology, Rabin Medical Center, Zeev Jabutinsky Rd 39, Petah- Tikva, Israel;6. Internal medicine “T”, Tel Aviv Sourasky Medical Center, Weizmann St 6, Tel Aviv, Israel;1. Department of Advanced Cardiovascular Therapeutics, Nagoya, Japan;2. Department of Cardiology, Nagoya University Graduate School of Medicine, Nagoya, Japan;1. Animal Genetics, University of Tübingen, Germany;2. Biozentrum, University of Würzburg, Germany;3. Max-Planck Institute for Developmental Biology, Tübingen, Germany;4. iBV, Université Nice Sophia-Antipolis, France;1. Laboratório de Cupins, Departamento de Biologia, Instituto de Biociências, UNESP – Univ Estadual Paulista, Av. 24A, No. 1515, 13506-900, Rio Claro, SP, Brazil;2. Instituto de Ciências Agrárias, Universidade Federal de Uberlândia – UFU, Av. Goiás, No. 2000, 38500-000, Monte Carmelo, MG, Brazil |
| |
| Abstract: | We developed a computer-based system for controlling the photoperiod and irradiance of UV-B and white light from a 5 × 5 light-emitting diode (LED) matrix (100 × 100 mm). In this system, the LED matrix was installed in each of four irradiation boxes and controlled by pulse-width modulators so that each box can independently emit UV-B and white light at irradiances of up to 1.5 and 4.0 W m−2, respectively, or a combination of both light types. We used this system to examine the hatchabilities of the eggs of four Tetranychus spider mite species (T. urticae, T. kanzawai, T. piercei and T. okinawanus) collected from Okinawa Island under UV-B irradiation alone or simultaneous irradiation with white light for 12 h d−1 at 25 °C. Although no eggs of any species hatched under the UV-B irradiation, even when the irradiance was as low as 0.02 W m−2, the hatchabilities increased to >90% under simultaneous irradiation with 4.0 W m−2 white light. At 0.06 W m−2 UV-B, T. okinawanus eggs hatched (15% hatchability) under simultaneous irradiation with white light, whereas other species showed hatchabilities <1%. These results suggest that photolyases activated by white light may reduce UV-B–induced DNA damage in spider mite eggs and that the greater UV-B tolerance of T. okinawanus may explain its dominance on plants in seashore environments, which have a higher risk of exposure to reflected UV-B even on the undersurface of leaves. Our system will be useful for further examination of photophysiological responses of tiny organisms because of its ability to precisely control radiation conditions. |
| |
| Keywords: | Irradiance Photolyase Photoreactivation Pulse-width modulator UV-B irradiation |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
