Role of Bacopa monnieri in the temporal regulation of oxidative stress in clock mutant (cryb) of Drosophila melanogaster |
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Affiliation: | 1. Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Chidambaram 608 002, Tamil Nadu, India;2. University of Malaya Centre for Proteomics Research (UMCPR), Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia;3. Department of Molecular Medicine, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia;1. Leonard Davis School of Gerontology, of the Ethel Percy Andrus Gerontology Center, the University of Southern California, Los Angeles, CA 90089-0191, USA;2. Molecular and Computational Biology Program, Department of Biological Sciences, of the Dornsife College of Letters, Arts, and Sciences, the University of Southern California, Los Angeles, CA 90089-0191, USA;1. Department of Food Protectants and Infestation Control, CSIR-Central Food Technological Research Institute, Mysore, India;2. Department of Zoology, Drosophila Neurobiology Laboratory, Nagaland University (Central), Lumami, India;3. Department of Biochemistry, CSIR-Central Food Technological Research Institute, Mysore, India |
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Abstract: | Accruing evidences imply that circadian organization of biochemical, endocrinological, cellular and physiological processes contribute to wellness of organisms and in the development of pathologies such as malignancy, sleep and endocrine disorders. Oxidative stress is known to mediate a number of diseases and it is notable to comprehend the orchestration of circadian clock of a model organism of circadian biology, Drosophila melanogaster, under oxidative stress. We investigated the nexus between circadian clock and oxidative stress susceptibility by exposing D. melanogaster to hydrogen peroxide (H2O2) or rotenone; the reversibility of rhythms following exposure to Bacopa monnieri extract (ayurvedic medicine rich in antioxidants) was also investigated. Abolishment of 24 h rhythms in physiological response (negative geotaxis), oxidative stress markers (protein carbonyl and thiobarbituric acid reactive substances) and antioxidants (superoxide dismutase, catalase, glutathione-S-transferase and reduced glutathione) were observed under oxidative stress. Furthermore, abolishment of per mRNA rhythm in H2O2 treated wild type flies and augmented susceptibility to oxidative stress in clock mutant (cryb) flies connotes the role of circadian clock in reactive oxygen species (ROS) homeostasis. Significant reversibility of rhythms was noted following B. monnieri treatment in wild type flies than cryb flies. Our experimental approach revealed a relationship involving oxidative stress and circadian clock in fruit fly and the utility of Drosophila model in screening putative antioxidative phytomedicines prior to their use in mammalian systems. |
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Keywords: | Antioxidant Circadian Cryptochrome |
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