A high involvement of O2- possibly generated in inner membranes for iron-induced microsomal lipid peroxidation. |
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Authors: | S Koga M Nakano |
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Institution: | Photon Medical Research Center, Hamamatsu University School of Medicine, Shizuoka, Japan. |
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Abstract: | The lipid peroxidation of and the O2- generation by rat liver microsomes in the presence of NADPH or both NADPH and Fe3+ were determined by thiobarbituric acid-reacting substance formation and by chemiluminescence intensities with a cypridina luciferin analog, 2-methyl-6-(p-methoxyphenyl)-3, 7-dihydroimidazo1,2-a]pyrazin-3-one(MCLA), as a chemiluminescence probe. Judging from the experiments with various inhibitors on the O2- generation and the lipid peroxidation, O2- generated, at intramembranous site, by cytochrome P-450 system is considered to be highly involved in the iron-induced lipid peroxidation. |
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