LncRNA NKILA inhibits the proliferation and promotes the apoptosis of CSCC cells by downregulating miRNA-21 |
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Authors: | Jinou Wang Zhi Zhu Hui Qiu Chang Liu Xiaoying Chang Yafei Qi Qingjie Lv |
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Institution: | 1. Department of Pathology, Shengjing Hospital of China Medical University, Shenyang, China;2. Department of Surgical Oncology, The First Hospital of China Medical University, Shenyang, China;3. Department of Gynaecology, Shengjing Hospital of China Medical University, Shenyang, China |
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Abstract: | Long noncoding RNA (lncRNA) NKILA has been well studied in several types of human tumors as a tumor suppressor, while its involvement in cervical squamous cell carcinoma (CSCC) remains unclear. In our studies, we found that serum NKILA was at lower levels and serum microRNA-21 (miRNA-21) was at higher levels in patients with early stage CSCC than in the healthy female. Altered expression of NKILA and miRNA-21 can effectively separate patients with CSCC at an early stage from healthy controls. Serum levels of NKILA were significantly and negatively correlated with miRNA-21 in patients with CSCC but not in normal controls. Overexpression of NKILA mediated the inhibited expression of miRNA-21 in CSCC cells, but mimic transfection of miRNA-21 did not significantly change the expression level of NKILA. Overexpression of NKILA repressed the proliferation and promoted the apoptosis of CSCC cells, while miRNA-21 showed opposite functions. In addition, miRNA-21 mimic transfection reduced the effects of NKILA on CSCC cells. Collectively, lncRNA NKILA could repress the proliferation and promote the apoptosis of CSCC cells by downregulating miRNA-21. |
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Keywords: | apoptosis cervical squamous cell carcinoma lncRNA NKILA miRNA-21 proliferation |
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