Muscle regeneration in adiponectin knockout mice showed early activation of anti-inflammatory response with perturbations in myogenesis |
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Authors: | Francielle C Mosele Rafael Bissi Ricci Phablo Abreu José C Rosa Neto |
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Institution: | 1. Department of Cellular Biology and Development, Institute of Biomedical Sciences, University of São Paulo (USP), São Paulo, Brazil;2. Department of Biochemistry, Institute of Chemistry, University of São Paulo (USP), São Paulo, Brazil |
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Abstract: | Activation, proliferation, and differentiation of satellite cells can be influenced by extracellular factors, such as adiponectin. This adipokine has been proposed as a regulator of in vitro myogenesis, but its action on in vivo regeneration is not still elucidated. We used C57BL/6 (wild-type WT]) and adiponectin knockout (AdKO) mice injured with barium chloride at periods of 3, 7, and 14 days after injury. The AdKO presented a higher number of centralized nuclei after 7 days, and a reduction in myogenic genes was observed after 3 days. Moreover, these mice presented an increase in anti-inflammatory cytokines after 3 and 7 days, and an increase in the M2 gene marker and proinflammatory cytokines after 7 days. The WT demonstrated an increase in adiponectin messenger RNA after 7 days. These results demonstrate that adiponectin is important in tissue remodeling during regeneration and that its deficiency does not compromise the maturation of muscle fibers, due to an increase in anti-inflammatory response; however, there is a possible impairment in proinflammatory response and an increase in centralized myonuclei. |
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Keywords: | adiponectin inflammation muscle regeneration markers satellite cell |
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