Retracted: HO-1 overexpression alleviates senescence by inducing autophagy via the mitochondrial route in human nucleus pulposus cells |
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Authors: | Weiwei Yi Haiyang Lan Yafeng Wen Yiyang Wang Danshuang He Zhibiao Bai Ye Zhang Wei Jiang Bo Liu Jieliang Shen Zhenming Hu |
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Institution: | Department of Orthopaedic Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China |
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Abstract: | Intervertebral disc degeneration (IDD) is closely associated with aging. Our previous studies have confirmed that heme oxygenase-1 (HO-1) can inhibit nucleus pulposus (NP) cell apoptosis. However, whether or not HO-1 is involved in NP cell senescence and autophagy is unclear. Our results indicated that HO-1 expression was reduced in IDD tissues and replicative senescent NP cells. HO-1 overexpression using a lentiviral vector reduced the NP cell senescence level, protected mitochondrial function, and promoted NP cell autophagy through the mitochondrial pathway. Autophagy inhibitor 3-MA pretreatment reversed the anti-senescent and protective effects on the mitochondrial function of HO-1, which promoted the degradation of the extracellular matrix (ECM) in the intervertebral disc. In vivo, HO-1 overexpression inhibited IDD and enhanced autophagy. In summary, these results suggested that HO-1 overexpression alleviates NP cell senescence by inducing autophagy via the mitochondrial route. |
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Keywords: | autophagy HO-1 mitochondrial nucleus pulposus cells senescence |
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