Toll-like receptors and high mobility group box 1 in granulosa cells during bovine follicle maturation |
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| Authors: | Yingying Xie Kang Zhang Kai Zhang Jingyan Zhang Lei Wang Xurong Wang Xuequan Hu Zijing Liang Jianxi Li |
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| Affiliation: | 1. Engineering & Technology Research Center of Traditional Chinese Veterinary Medicine of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou, China;2. College of Veterinary Medicine, Gansu Agricultural University, Lanzhou, China |
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| Abstract: | Toll-like receptors (TLRs) are present in the ovaries and reproductive tract of various mammals. The biological function of TLR during ovulation is one of the main contents in the research of reproductive immunology. In this study, we found that messenger RNA levels of TLR1–TLR10 in granulosa cells were different, and TLRs and high mobility group box 1 (HMGB1) in granulosa cells of large follicles were significantly higher than those of small and middle follicles. Coimmunoprecipitation results showed that HMGB1 interacts with TLR2 in granulosa cells, especially large follicles. The result of immunohistochemistry showed that TLRs and HMGB1 were present in granulosa cell layer of ovarian follicles. We also found 25 mIU/ml follicle-stimulating hormone (FSH) significantly upregulated the expression of TLRs and HMGB1. These results suggest that TLR2/4 and HMGB1 in granulosa cells may be involved in the ovarian innate immune and ovarian follicular maturation, regulated by FSH. However, further research of the function and mechanisms of TLRs and HMGB1 in granulosa cells are needed. |
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| Keywords: | bovine FSH HMGB1 ovarian granulosa cells TLR2 TLR4 |
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