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Circular RNA hsa_circRNA_103809 promoted hepatocellular carcinoma development by regulating miR-377-3p/FGFR1/ERK axis |
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| Authors: | Wei Zhan Xin Liao Zhongsheng Chen Lianghe Li Tian Tian Lei Yu Wei Wang Qiyan Hu |
| Affiliation: | 1. Department of Colorectal Surgery, Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou, China Wei Zhan and Xin Lia contributed equally to this study.;2. Department of Imaging, Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou, China Wei Zhan and Xin Lia contributed equally to this study.;3. Clinical Medical College, Guizhou Medical University, Guiyang, Guizhou, China;4. Centre of Clinical Laboratory, Guiyang Maternal and Child Health Hospital, Guiyang City, Guizhou, China;5. Department of Pathology, Guiyang Maternal and Child Health Hospital, Guiyang, Guizhou, China;6. Department of Gastroenterology, Xiangyang Central Hospital, Affiliated Hospital of Hubei University of Arts and Science, Xiangyang, Hubei, China;7. Department of Oncology, Xiangyang Central Hospital, Affiliated Hospital of Hubei University of Arts and Science, Xiangyang, Hubei, China |
| Abstract: | In the last decade, circular RNAs (circRNAs) emerge as important regulators in multiple biological processes. Lately, it is reported hsa_circRNA_103809 could play vital parts in several types of cancers. Based on the analysis of GEO data (GSE97332), hsa_circRNA_103809 was found to be dysregulated in hepatocellular carcinoma (HCC). However, the biological function and underlying regulatory mechanisms of hsa_circRNA_103809 in HCC remain unclear. Our results suggested that hsa_circRNA_103809 was overexpressed in HCC patients, and hsa_circRNA_103809 knockdown remarkably inhibited the proliferation, cycle progression, and migration of HCC cells. The investigations of molecular showed that hsa_circRNA_103809 could elevate the protein expression of a miR-377-3p target, fibroblast growth factor receptor 1 (FGFR1), through interacting with miR-377-3p and decreasing its expression level. Additionally, in vivo assays revealed hsa_circRNA_103809 short hairpin RNA served as a tumor suppressor through downregulating FGFR1 in HCC. This study systematically investigated novel regulatory signaling of hsa_circRNA_103809/miR-377-3p/FGFR1 axis, providing insights into hepatocellular carcinoma treatment from bench to clinic. |
| Keywords: | cell cycle cell migration cell proliferation FGFR1 hepatocellular carcinoma hsa_circRNA_103809 miR-377-3p |