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Laminin-derived peptide C16 regulates Tks expression and reactive oxygen species generation in human prostate cancer cells |
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| Authors: | Livia Caires-dos-Santos Suély V. da Silva Basilio Smuczek Adriane S. de Siqueira Karen S. P. Cruz José Alexandre M. Barbuto Taize M. Augusto Vanessa M. Freitas Hernandes F. Carvalho Ruy G. Jaeger |
| Affiliation: | 1. Department of Cell and Developmental Biology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, SP, Brazil Livia Caires-dos-Santos, Suély V. da Silva, and Basilio Smuczek contributed equally to this study.;2. Department of Cell and Developmental Biology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, SP, Brazil Department of Biology, UNICENTRO State University, Guarapuava, PR, Brazil Livia Caires-dos-Santos, Suély V. da Silva, and Basilio Smuczek contributed equally to this study.;3. Department of Cell and Developmental Biology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, SP, Brazil School of Dentistry, Positivo University, Curitiba, PR, Brazil;4. Department of Immunology, Institute of Biomedical Sciences, University of Sao Paulo, Sao Paulo, SP, Brazil Cell Biology Laboratory, Institute of Health and Biological Sciences, Federal University of Alagoas, Maceio, AL, Brazil Faculty of Nutrition, Federal University of Alagoas, Maceio, AL, Brazil;5. Department of Immunology, Institute of Biomedical Sciences, University of Sao Paulo, Sao Paulo, SP, Brazil;6. Department of Structural and Functional Biology, Institute of Biology, University of Campinas, Campinas, SP, Brazil Department of Morphology and Basic Pathology, School of Medicine of Jundiai, Jundiai, SP, Brazil;7. Department of Cell and Developmental Biology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, SP, Brazil;8. School of Dentistry, Positivo University, Curitiba, PR, Brazil |
| Abstract: | Laminin peptides influence cancer biology. We investigated the role of a laminin-derived peptide C16 regulating invadopodia molecules in human prostate cancer cells (DU145). C16 augmented invadopodia activity of DU145 cells, and stimulated expression Tks4, Tks5, cortactin, and membrane-type matrix metalloproteinase 1. Reactive oxygen species generation is also related to invadopodia formation. This prompted us to address whether C16 would induce reactive oxygen species generation in DU145 cells. Quantitative fluorescence and flow cytometry showed that the peptide C16 increased reactive oxygen species in DU145 cells. Furthermore, significant colocalization between Tks5 and reactive oxygen species was observed in C16-treated cells. Results suggested that the peptide C16 increased Tks5 and reactive oxygen species in prostate cancer cells. The role of C16 increasing Tks and reactive oxygen species are novel findings on invadopodia activity. |
| Keywords: | extracellular matrix invadopodia laminin prostate cancer reactive oxygen species Tks4 Tks5 |