Sizes and mass distributions of clathrin-coated vesicles from bovine brain |
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Authors: | R Nossal G H Weiss P K Nandi R E Lippoldt H Edelhoch |
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Affiliation: | 1. Physical Sciences Laboratory, DCRT, National Institutes of Health, Bethesda, Maryland 20205 USA;2. Clinical Endocrinology Branch, NIADDK, National Institutes of Health, Bethesda, Maryland 20205 USA |
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Abstract: | Clathrin-coated vesicles obtained from bovine brain have been studied by ultracentrifugation and dynamic light scattering techniques to provide information on their sedimentation and mass distributions and their average diffusion coefficients. "Uncoated" vesicles, obtained by removing the protein coat from coated vesicles, have been similarly characterized. For typical preparations, maximal values of approximately 210 and 95 S are observed for the sedimentation coefficients of coated and uncoated vesicles, respectively. Corresponding values for the average molecular weights, determined from values of average sedimentation and diffusion coefficients, are 49 X 10(6) and 13 X 10(6); values obtained by equilibrium sedimentation are 37.2 X 10(6) and 10.6 X 10(6). In order to obtain these results, some minor modifications of sedimentation and light-scattering techniques have been devised which may have application to other studies of size distributions of large particles. |
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Keywords: | To whom correspondence should be addressed: CEB NIADDK NIH Bldg. 10 Room 8N/310 Bethesda Md 20205. |
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