Biochemical and immunochemical characterization of two simian virus 40 (SV40)-specific glycoproteins in nuclear and surface membranes of SV40-transformed cells.

Plasma membranes of several simian virus 40-transformed cells contain virus-specific proteins with molecular masses of ～ 100,000D and ～ 60,000D and isoelectric points of ～ 4.7 and ～ 4.5, respectively. Triton X-100 extracts of purified nuclei from simian virus 40-transformed hamster lymphocytes contain the same proteins but in different proportions, the high molecular mass component being enriched six-fold in terms of the lower molecular mass one. Both proteins can be labeled metabolically with ¹⁴C]glucosamine and their isoelectric points altered by neuraminidase treatment, showing that they are sialoglycoproteins.