Molecular cloning and characterization of a cDNA encoding ent-cassa-12,15-diene synthase, a putative diterpenoid phytoalexin biosynthetic enzyme, from suspension-cultured rice cells treated with a chitin elicitor |
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Authors: | Cho Eun-Min Okada Atsushi Kenmoku Hiromichi Otomo Kazuko Toyomasu Tomonobu Mitsuhashi Wataru Sassa Takeshi Yajima Arata Yabuta Goro Mori Kenji Oikawa Hideaki Toshima Hiroaki Shibuya Naoto Nojiri Hideaki Omori Toshio Nishiyama Makoto Yamane Hisakazu |
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Affiliation: | Biotechnology Research Center, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan. |
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Abstract: | We have isolated and characterized a cDNA encoding a novel diterpene cyclase, OsDTC1, from suspension-cultured rice cells treated with a chitin elicitor. OsDTC1 functions as ent-cassa-12,15-diene synthase, which is considered to play a key role in the biosynthesis of (-)-phytocassanes recently isolated as rice diterpenoid phytoalexins. The expression of OsDTC1 mRNA was also confirmed in ultraviolet (UV)-irradiated rice leaves. In addition, we identified ent-cassa-12,15-diene, a putative diterpene hydrocarbon precursor of (-)-phytocassanes, as an endogenous compound in the chitin-elicited suspension-cultured rice cells and the UV-irradiated rice leaves. The OsDTC1 cDNA isolated here will be a useful tool to investigate the regulatory mechanisms of the biosynthesis of (-)-phytocassanes in rice. |
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Keywords: | rice phytoalexin diterpene cyclase (−)-phytocassane chitin elicitor suspension-cultured cell |
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