Use of viral replicons for the expression of genes in plants |
| |
Authors: | Claudine Porta George P Lomonossoff |
| |
Institution: | (1) Department of Virus Research, John Innes Centre, Colney Lane, NR4 7UH Norwich, UK |
| |
Abstract: | Autonomously replicating virus-based vectors have been investigated as a means of introducing heterologous genes into plants.
This approach has a number of potential advantages over stable genetic transformation, particularly in terms of speed and
levels of expression that can be obtained. Several groups of plant viruses, with genomes consisting of both DNA and RNA, have
been investigated as possible gene vectors. In the case of DNA viruses, it has generally been possible to identify nonessential
regions of the genome that can be replaced by foreign sequences. However, there appear to be limitations on the size of insert
which can be tolerated. In the case of RNA viruses, replacement of viral sequences usually has a drastic effect on the viability.
However, in several cases it has proved possible to substantially increase the size of the viral genome by the direct insertion
of additional sequences while still retaining the ability of the viruses to multiply and spread in plants. These RNA virus-based
systems appear to have the greatest potential as gene vectors. |
| |
Keywords: | Plant virus vector gene transfer chimeric particles protein engineering |
本文献已被 SpringerLink 等数据库收录! |
|