<Emphasis Type="Italic">In vitro</Emphasis> and <Emphasis Type="Italic">in vivo</Emphasis> assays for osteoclast apoptosis |
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Authors: | Toru?Akiyama Tsuyoshi?Miyazaki Phillippe?Bouillet Kozo?Nakamura Andreas?Strasser Email author" target="_blank">Sakae?TanakaEmail author |
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Affiliation: | (1) Department of Orthopaedic Surgery, Faculty of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, 113-0033 Tokyo, Japan;(2) The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, 3050 Parkville, Victoria, Australia |
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Abstract: | Mature osteoclasts, multinucleated giant cells responsible for bone resorption, are terminally differentiated cells with a
short life span. Recently, we have demonstrated that osteoclast apoptosis is regulated by ERK activity and Bcl-2 family member
Bim. In this paper, we summarize the methods we used to study osteoclast apoptosis in vitro and in vivo. Using adenovirus and retrovirus vectors, we were able to introduce foreign genes into osteoclasts and examine their effects
on osteoclast survival in vitro. In addition, we established the modified methods for in situ hybridization and BrdU labeling of bone sections from mice
to study osteoclast survival in vivo. The detailed methods described here could be useful for studying the biological process in bone. |
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Keywords: | Indexing terms" target="_blank">Indexing terms Apoptosis Macrophage Colony-Stimulating Factor Osteoclasts |
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