Intragenomic diversity of the V1 regions of 16S rRNA genes in high-alkaline protease-producing Bacillus clausii spp |
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| Authors: | Yasushi Kageyama Yoshihiro Takaki Shigeru Shimamura Shinro Nishi Yuichi Nogi Kohsuke Uchimura Tohru Kobayashi Jun Hitomi Katsuya Ozaki Shuji Kawai Susumu Ito Koki Horikoshi |
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| Affiliation: | (1) Tochigi Research Laboratories of Kao Corporation, 2606 Akabane, Ichikai, Haga, Tochigi 321-3497, Japan;(2) Japan Agency for Marine-Earth Science and Technology (JAMSTEC), 2-15 Natsushima, Yokosuka 237-0061, Japan;(3) Creative Research Initiative “Sousei” (CRIS), Hokkaido University, North-21, West-10, Sapporo 001-0021, Japan |
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| Abstract: | Alkaliphilic Bacillus sp. strain KSM-K16, which produces high-alkaline M-protease, was characterized phenotypically, biochemically and genetically. This strain was identified as Bacillus clausii based on the results of taxonomic studies, including sequencing of the 16S rRNA gene and DNA-DNA hybridization. Seven rRNA operons in the genome were identified by pulsed-field gel electrophoresis. Sequencing of cloned 16S rRNA genes revealed two distinct types of variable region V1. Moreover, some cloned 16S rRNA genes in some of the reference strains of B. clausii had a V1 region of yet another type. The B. clausii strains could clearly be divided into at least two subgroups based on the frequencies of the types of cloned V1 sequence. Bacillus sp. strain KSM-K16 was found to be in a different phylogenetic position from other high-alkaline protease-producing strains of B. clausii. |
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| Keywords: | High-alkaline protease Subtilisin Bacillus clausii 16S rRNA Intragenomic variation Variable region |
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