Absence of genotoxic effects of nonasbestos mineral fibers |
| |
Authors: | Francine Denizeau Michel Marion Gaston Chevalier Michel G Cote |
| |
Institution: | (1) Département de chimie, Université du Québec a Montréal, Montréal, Canada;(2) Département de chimie, Université du Quebec a Montréal, C.P. 8888, Succ. A, Montréal, Canada, H3C 3P8;(3) Département des sciences biologiques, Université du Québec a Montréal, Montréal, Canada;(4) Département de pharmacologie, Université de Montréal, Montréal, Canada |
| |
Abstract: | The biological activity of natural and synthetic mineral fibers has been examined. Natural attapulgite (Mg, Al)2Si4O10(OH).4H20], synthetic xonotlite Ca3Si3O8(OH)2] and natural sepiolite Mg2Si3O8.2H2O] were selected. Genotoxic effects were investigated by means of a well established cellular model based upon the measurement of unscheduled DNA synthesis (UDS) in rat hepatocytes in primary culture. The intrinsic capacity of the fibers (1 and 10 µ/ml) to induce UDS was first tested. None of the fiber types showed detectable UDS-eliciting activity. Also, the possible modulation of the cellular response to genotoxic agents by the materials was examined by exposing the cells to mixtures of 2-acetylaminofluorene (AAF) (0.05 and 0.25 µg/ml) and fibers (1 and 10 µg/ml). In these experiments, the UDS response was significantly diminished in the presence of xonotlite. This phenomenon may reflect changes in the uptake and/or metabolism of AAF or may result from an inhibition of DNA repair processes, the latter suggesting a possible cocarcinogenic potential for this synthetic silicate. These results point to the immediate necessity of studying more extensively the biological effects of fibrous materials that can be used as substitutes for asbestos.Abbreviations AAF
2-acetylaminofluorene
- DMSO
dimethyl-sulfoxide
- FBS
fetal bovine serum
- IRDA
Institut de Recherche et de Développement sur l'Amiante
- LDH
lactate dehydrogenase
- UDS
unscheduled
- DNA
synthesis
- WME
Williams' Medium E
This work was supported by the Institut de Recherche et de Développement sur l'Amiante (IRDA), Sherbrooke, Canada. |
| |
Keywords: | genotoxicity UDS hepatocytes attapulgite xonotlite sepiolite |
本文献已被 SpringerLink 等数据库收录! |
|