Novel aliphatic epoxide hydrolase activities from dematiaceous fungi |
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| Authors: | Gideon Grogan Stanley M Roberts rew J Willetts |
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| Institution: | Department of Biological Sciences, Washington Singer Laboratories, University of Exeter, Exeter, Devon EX4 4QG, UK; Department of Chemistry, University of Liverpool, Liverpool L69 3BX, UK |
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| Abstract: | Abstract Epoxide hydrolases were found to be constitutively expressed in dematiaceous fungi coincident with secondary metabolite pigment production in stationary or idiophase. Washed-cell preparations of two fungi, Ulodadium atrum CMC 3280 and Zopfiella karachiensis CMC 3284, exhibited affinity for 2,2-dialkylated oxiranes, for which contrasting enantioselectivities were observed, but not for aromatic styrene oxide or alicyclic cyclohexene oxide type substrates. Lyophilised preparations of soluble epoxide hydrolase activities proved to be effective catalysts for the mild hydrolysis of aliphatic epoxides. |
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| Keywords: | Dematiaceous fungi Biotransformation Chiral epoxide Epoxide hydrolase |
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