Identification of two plasmids determining resistance to tetracycline and to erythromycin in group D streptococcus |
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Authors: | P M Courvalin C Carlier O Croissant and D Blangy |
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Institution: | (1) Départment de Bactériologie médicale, Institut Pasteur, Paris, France;(2) Départment de Virologie, Institut Pasteur, Paris, France;(3) Départment de Biologie moléculaire, Institut Pasteur, Paris, France |
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Abstract: | Summary Two circular DNA plasmids designated Tet and Ero, distinguishable by their molecular weights (64.5 and 17.6×106 respectively) and their guanine plus cytosine (G+C) content (36.5 and 34.5% respectively) were identified in Streptococcus faecalis strain KR, resistant to tetracycline and erythromycin. Loss of resistance to tetracycline, to erythromycin and to both antibiotics simultaneously in independently isolated cured derivatives corresponds to the loss of the Tet, Ero, and of both plasmids respectively. In the singly resistant strains we estimated that 3 and 6 copies of the Tet and Ero plasmids respectively are present per chromosomal genome equivalent.Abbreviations form I
supercoiled circular DNA
- form II
relaxed circular DNA
- form III
linear double-strand DNA
- Tet
tetracycline
- Ero
erythromycin
- R
resistant
- S
sensitive |
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Keywords: | |
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