Synthesis of poly(A) containing RNA induced by ecdysterone in fat body cells of Calliphora vicina |
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Authors: | Klaus Scheller Peter Karlson |
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Institution: | Physiologisch-Chemisches Institut, der Philipps-Universität, Deutschhausstr. 1–2, D-3550 Marburg. G.F.R. |
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Abstract: | The insect-hormone ecdysterone causes a de novo synthesis of RNA in the fat body cells of blowfly larvae. Hybridization of the RNA induced by the hormone with 3H-Poly(U) demonstrates that it contains poly(A) sequences and therefore is probably informational. DNA-RNA-hybridization experiments further show that qualitatively new mRNA species are synthesized under the influence of ecdysterone. The inducibility of RNA synthesis is strongly correlated to specific developmental stages. |
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