Molecular Characterization of Extrachromosomal DNA Accompanying Primula Red Phytoplasma |
| |
Authors: | Tatiana Sarkisova Ondřej Lenz Karel Petrzik |
| |
Affiliation: | Department of Plant Virology, Institute of Plant Molecular Biology, Biology Centre ASCR, ?eské Budějovice, Czech Republic |
| |
Abstract: | The complete nucleotide sequence of an extrachromosomal element found in primula red isolate of ‘Candidatus Phytoplasma asteris’ (16SrI‐B subgroup) was determined. The plasmid, named pPrR, is 4378 bp in length and has 75% A+T content that is similar to that of the phytoplasma genome. It encodes six putative open reading frames (ORF) longer than 100 amino acids and two smaller ones. The structural organization of the rep gene is similar to that found in plasmids which replicate via rolling circle mechanism. Furthermore, it has homology to both the plasmid pLS1 family and helicase domains of replication‐associated proteins (Rap) of eukaryotic viruses and geminiviruses. The ORF arrangement and genes sequences are most similar to the pPARG1 plasmid from ‘Rehmannia glutinosa’ phytoplasma. |
| |
Keywords: | phylogenetic analysis phytoplasma plasmid rep gene |
|
|