The genetic characterisation of novel multi-addition doubled haploid lines derived from triticale x wheat hybrids |
| |
Authors: | G. Wang J. Ji Y-B. Wang H. Hu I. P. King J. W. Snape |
| |
Affiliation: | (1) Institute of Genetics, Academia Sinica, 100101 Beijing, China;(2) JI Centre for Plant Science Research, Cambridge Laboratory, Colney Lane, NR4 7UJ Norwich, England |
| |
Abstract: | Two novel 46-chromosome doubled haploid lines, W66 and M17, derived from separate hexaploid triticale x bread wheat crosses, were characterised using cytological and biochemical markers. Both lines were shown to be relatively stable cytologically, over 11 and 8 generations of selfing, respectively. By examining mitotic and meiotic chromosomes, the stabilities of the two lines were shown to be similar with frequencies of 2n=46 in 74.2–85.5% of cells. However, over selfed generations, the rye chromosomes were shown to have lost some of their heterochromatin, which made it difficult to establish their continued presence using cytological techniques, such as C-banding alone. Cytological evidence from pairing studies, C-banding, and fluorescence in-situ hybridization, showed that both M17 and W66 are wheat/rye multi-addition lines with rye chromosome constitutions of 1R+6R, and 1R+4R, respectively. These conclusions were confirmed by isozyme and storage-protein analysis. |
| |
Keywords: | Triticale x wheat Doubled haploids Biochemical markers In situ hybridization |
本文献已被 SpringerLink 等数据库收录! |
|