兔出血症病毒细胞培养的初步研究

A PRELIMINARY REPORT ON THE PROPAGATION OF RABBIT HAEMORRHAGIC DISEASE VIRUS IN CELL CULTURE

Liver emulsion of rabbits which died of rabbit haemorrhagic disease ( RHD ) was inoculated onto DJRK cells, a continuous cell line established from rabbit kidney cells. After 3 passages, the CPE was rather regular, most of the cells became round,aggregated and finally detached. Im munofluorescence was mainly found in the cytoplasm around the nucleus by indirect immunofluorescent assay.The 5th passage cell culture virus was inoculated into 8 susceptible rabbits. Viral HA was detected in all serum samples and lasted for several days. All rabbits died at day 5-11 postinoculation. The pathological changes were typical of RHD. Similar results were obtained with the 10th and 16th passage materials.It was shown by electronmicroscopy that there were numerous virions in the cells infected with the virus of 10th passage, 30-34nm in diameter, mainly accumulated around the nuclei, and some in the nuclei.The 11th passage cell culture virus inactivated with formaldehyde was inoculated into healthy rabbits. Very high HI antibody was produced and the rabbits could resist challenge with viurlent RHDV.