Mineralisation of 14C-labelled synthetic lignin and ligninolytic enzyme activities of litter-decomposing basidiomycetous fungi |
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Authors: | K T Steffen M Hofrichter A Hatakka |
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Institution: | (1) Department of Applied Chemistry and Microbiology, P.O. Box 56, 00014 University of Helsinki, Helsinki, Finland e-mail: Kari.Steffen@Helsinki.Fi Fax: +358-9-19159322, FI |
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Abstract: | Within a screening program, 27 soil litter-decomposing basidiomycetes were tested for ligninolytic enzyme activities using
agar-media containing 2,2′-azinobis(3-ethylbenzthiazoline-6-sulphonate), a humic acid or Mn2+ ions as indicator substrates. Most active species were found within the family Strophariaceae (Agrocybe praecox, Stropharia coronilla, S. rugosoannulata) and used for mineralisation experiments with a 14C-ring-labelled synthetic lignin (14C-DHP). The fungi mineralised around 25% of the lignin to 14CO2 within 12 weeks of incubation in a straw environment; about 20% of the lignin was converted to water-soluble fragments. Mn-peroxidase
was found to be the predominant ligninolytic enzyme of all three fungi in liquid culture and its production was strongly enhanced
in the presence of Mn2+ ions. The results of this study demonstrate that certain ubiquitous litter-decomposing basidiomycetes possess ligninolytic
activities similar to the wood-decaying white-rot fungi, the most efficient lignin degraders in nature.
Received: 20 April 2000 / Received revision: 12 July 2000 / Accepted: 16 July 2000 |
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