Crystalline enzyme kinetics: activity of the Streptomyces R61 D-alanyl-D-alanine peptidase. |
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| Authors: | J A Kelly S G Waley M Adam J M Frère |
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| Affiliation: | Sir William Dunn School of Pathology, University of Oxford, U.K. |
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| Abstract: | The specificity constant, kcat/Km, for the hydrolysis of hippuryl-mercaptoacetate by crystals of the Streptomyces R61 D-D peptidase was measured by reaction of the thiol produced with 4,4'-dithiodipyridine. The values of kcat/Km for the crystal and in solution were the same (within experimental error). A novel method for treating the lag in the progress curves was developed. |
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